Peña Madridista De Boada La Novena

dos.5. California dos+ imaging within the neonatal OHCs

dos.5. California dos+ imaging within the neonatal OHCs The brand new rodent Ocm gene (succession within the Shape S01) try cloned towards the pcDNA3.1CFP and pEGFP-N1 Flag (Addgene #60360). Then, EGFP-Flag was removed playing with BamHI (NEB) and NotI (NEB) and replaced with new mCherry succession (gene stop off mCherry out-of Qiagen) to help make […]